| Title | Production and characterization of protective human antibodies against Shiga toxin 1. |
| Publication Type | Journal Article |
| Year of Publication | 2002 |
| Authors | Mukherjee J, Chios K, Fishwild D, Hudson D, O'Donnell S, Rich SM, Donohue-Rolfe A, Tzipori S |
| Journal | Infect Immun |
| Volume | 70 |
| Issue | 10 |
| Pagination | 5896-9 |
| Date Published | 2002 Oct |
| ISSN | 0019-9567 |
| Keywords | Animals, Antibodies, Bacterial, Antibodies, Monoclonal, Child, Escherichia coli, Female, HeLa Cells, Hemolytic-Uremic Syndrome, Humans, Hybridomas, Immunization, Passive, Immunoglobulin G, Immunoglobulin M, Mice, Neutralization Tests, Shiga Toxin 1 |
| Abstract | Hemolytic-uremic syndrome (HUS) is a serious complication which is predominantly associated in children with infection by Shiga toxin-producing Escherichia coli (STEC). By using HuMAb-Mouse (Medarex) animals, human monoclonal antibodies (Hu-MAbs) were developed against Shiga toxin 1 (Stx1) for passive immunotherapy of HUS. Ten stable hybridomas comprised of fully human heavy- and light-chain immunoglobulin elements and secreting Stx1-specific Hu-MAbs (seven immunoglobulin M(kappa)() [IgM(kappa)] elements [one specific for the A subunit and six specific for the B subunit] and three IgG1(kappa) elements specific for subunit B) were isolated. Two IgM(kappa) Hu-MAbs (2D9 and 15G9) and three IgG1(kappa) Hu-MAbs (5A4, 10F4, and 15G2), all specific for subunit B, demonstrated marked neutralization of Stx1 in vitro and significant prolongation of survival in a murine model of Stx1 toxicosis. |
| Alternate Journal | Infect. Immun. |
| PubMed ID | 12228326 |
Department of Microbiology