Title | Molecular analysis of the recF gene of Escherichia coli. |
Publication Type | Journal Article |
Year of Publication | 1984 |
Authors | Blanar MA, Sandler SJ, Armengod ME, Ream LW, Clark AJ |
Journal | Proc Natl Acad Sci U S A |
Volume | 81 |
Issue | 15 |
Pagination | 4622-6 |
Date Published | 1984 Aug |
ISSN | 0027-8424 |
Keywords | Amino Acid Sequence, Bacterial Proteins, Base Sequence, DNA Repair, DNA Replication, Escherichia coli, Genes, Bacterial, Molecular Weight, Mutation, Plasmids, Recombination, Genetic |
Abstract | We analyzed the nucleotide sequence of a 1.325-kilobase region of wild-type Escherichia coli containing a functional recF gene and six Tn3 mutations that inactivate recF. The analysis shows a potentially translatable reading frame of 1071 nucleotides, which is interrupted by all six insertions. A protein of 40.5 kilodaltons would result from translation of the open reading frame, and a radioactive band of protein of an apparent molecular weight of approximately 40 kilodaltons was seen by the maxicell method using a recF+ plasmid. Putative truncated peptides were seen when two recF::Tn3 mutant plasmids were used. Differential expression of dnaN and recF from a common promoter was noted. recF332::Tn3 was transferred to the chromosome where, in hemizygous condition, it produced UV sensitivity indistinguishable from that produced by two presumed recF point mutations. |
Alternate Journal | Proc. Natl. Acad. Sci. U.S.A. |
PubMed ID | 6379647 |
Department of Microbiology