|Imaging bacterial peptidoglycan with near-infrared fluorogenic azide probes.
|Year of Publication
|Shieh P, M Siegrist S, Cullen AJ, Bertozzi CR
|Proc Natl Acad Sci U S A
|2014 Apr 15
|Azides, Fluorescent Dyes, Molecular Imaging, Molecular Probe Techniques, Molecular Structure, Peptidoglycan
Fluorescent probes designed for activation by bioorthogonal chemistry have enabled the visualization of biomolecules in living systems. Such activatable probes with near-infrared (NIR) emission would be ideal for in vivo imaging but have proven difficult to engineer. We present the development of NIR fluorogenic azide probes based on the Si-rhodamine scaffold that undergo a fluorescence enhancement of up to 48-fold upon reaction with terminal or strained alkynes. We used the probes for mammalian cell surface imaging and, in conjunction with a new class of cyclooctyne D-amino acids, for visualization of bacterial peptidoglycan without the need to wash away unreacted probe.
|Proc. Natl. Acad. Sci. U.S.A.
|PubMed Central ID
|AI051622 / AI / NIAID NIH HHS / United States
GM058867 / GM / NIGMS NIH HHS / United States
R01 AI051622 / AI / NIAID NIH HHS / United States
R37 GM058867 / GM / NIGMS NIH HHS / United States
/ / Howard Hughes Medical Institute / United States