<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Swarts, Benjamin M</style></author><author><style face="normal" font="default" size="100%">Holsclaw, Cynthia M</style></author><author><style face="normal" font="default" size="100%">Jewett, John C</style></author><author><style face="normal" font="default" size="100%">Alber, Marina</style></author><author><style face="normal" font="default" size="100%">Fox, Douglas M</style></author><author><style face="normal" font="default" size="100%">Siegrist, M Sloan</style></author><author><style face="normal" font="default" size="100%">Leary, Julie A</style></author><author><style face="normal" font="default" size="100%">Kalscheuer, Rainer</style></author><author><style face="normal" font="default" size="100%">Bertozzi, Carolyn R</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Probing the mycobacterial trehalome with bioorthogonal chemistry.</style></title><secondary-title><style face="normal" font="default" size="100%">J Am Chem Soc</style></secondary-title><alt-title><style face="normal" font="default" size="100%">J. Am. Chem. Soc.</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Alkynes</style></keyword><keyword><style  face="normal" font="default" size="100%">Azides</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorescent Dyes</style></keyword><keyword><style  face="normal" font="default" size="100%">Glycolipids</style></keyword><keyword><style  face="normal" font="default" size="100%">Mycobacterium</style></keyword><keyword><style  face="normal" font="default" size="100%">Trehalose</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2012 Oct 3</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">134</style></volume><pages><style face="normal" font="default" size="100%">16123-6</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Mycobacteria, including the pathogen Mycobacterium tuberculosis, use the non-mammalian disaccharide trehalose as a precursor for essential cell-wall glycolipids and other metabolites. Here we describe a strategy for exploiting trehalose metabolic pathways to label glycolipids in mycobacteria with azide-modified trehalose (TreAz) analogues. Subsequent bioorthogonal ligation with alkyne-functionalized probes enabled detection and visualization of cell-surface glycolipids. Characterization of the metabolic fates of four TreAz analogues revealed unique labeling routes that can be harnessed for pathway-targeted investigation of the mycobacterial trehalome.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">39</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/22978752?dopt=Abstract</style></custom1></record></records></xml>