@article {3063,
	title = {Correlation of Key Physiological Properties of  Isolates with Environment of Origin.},
	journal = {Appl Environ Microbiol},
	volume = {87},
	year = {2021},
	month = {2021 Jun 11},
	pages = {e0073121},
	abstract = {<p>It is known that the physiology of  species can differ significantly, but the ecological impact of these differences is unclear. We recovered two strains of  from two different ecosystems with a similar enrichment and isolation method. Both strains had the same ability to metabolize organic substrates and participate in direct interspecies electron transfer but also had major physiological differences. Strain DH-1, which was isolated from an anaerobic digester, used H as an electron donor. Genome analysis indicated that it lacks an Rnf complex and conserves energy from acetate metabolism via intracellular H cycling. In contrast, strain DH-2, a subsurface isolate, lacks hydrogenases required for H uptake and cycling and has an Rnf complex for energy conservation when growing on acetate. Further analysis of the genomes of previously described isolates, as well as phylogenetic and metagenomic data on uncultured  in anaerobic digesters and diverse soils and sediments, revealed a physiological dichotomy that corresponded with environment of origin. The physiology of type I  revolves around H production and consumption. In contrast, type II  species eschew H and have genes for an Rnf complex and the multiheme, membrane-bound -type cytochrome MmcA, shown to be essential for extracellular electron transfer. The distribution of  species in diverse environments suggests that the type I H-based physiology is well suited for high-energy environments, like anaerobic digesters, whereas type II Rnf/cytochrome-based physiology is an adaptation to the slower, steady-state carbon and electron fluxes common in organic-poor anaerobic soils and sediments.  Biogenic methane is a significant greenhouse gas, and the conversion of organic wastes to methane is an important bioenergy process.  species play an important role in methane production in many methanogenic soils and sediments as well as anaerobic waste digesters. The studies reported here emphasize that the genus  is composed of two physiologically distinct groups. This is important to recognize when interpreting the role of  in methanogenic environments, especially regarding H metabolism. Furthermore, the finding that type I  species predominate in environments with high rates of carbon and electron flux and that type II  species predominate in lower-energy environments suggests that evaluating the relative abundance of type I and type II  may provide further insights into rates of carbon and electron flux in methanogenic environments.</p>},
	keywords = {Acetates, Anaerobiosis, Bioreactors, Ecosystem, Electron Transport, Ethanol, Genome, Archaeal, Hydrogen, Methane, Methanosarcina, Phylogeny},
	issn = {1098-5336},
	doi = {10.1128/AEM.00731-21},
	author = {Zhou, Jinjie and Holmes, Dawn E and Tang, Hai-Yan and Lovley, Derek R}
}
@article {1207,
	title = {Complete genome sequence of the hyperthermophilic archaeon Pyrococcus sp. strain ST04, isolated from a deep-sea hydrothermal sulfide chimney on the Juan de Fuca Ridge.},
	journal = {J Bacteriol},
	volume = {194},
	year = {2012},
	month = {2012 Aug},
	pages = {4434-5},
	abstract = {<p>Pyrococcus sp. strain ST04 is a hyperthermophilic, anaerobic, and heterotrophic archaeon isolated from a deep-sea hydrothermal sulfide chimney on the Endeavour Segment of the Juan de Fuca Ridge in the northeastern Pacific Ocean. To further understand the distinct characteristics of this archaeon at the genome level (polysaccharide utilization at high temperature and ATP generation by a Na(+) gradient), the genome of strain ST04 was completely sequenced and analyzed. Here, we present the complete genome sequence analysis results of Pyrococcus sp. ST04 and report the major findings from the genome annotation, with a focus on its saccharolytic and metabolite production potential.</p>},
	keywords = {Adenosine Triphosphate, Anaerobiosis, DNA, Archaeal, Genome, Archaeal, Heterotrophic Processes, Hydrothermal Vents, Molecular Sequence Data, Pacific Ocean, Polysaccharides, Pyrococcus, Seawater, Sequence Analysis, DNA, Sodium Chloride, Sulfides},
	issn = {1098-5530},
	doi = {10.1128/JB.00824-12},
	author = {Jung, Jong-Hyun and Lee, Ju-Hoon and Holden, James F and Seo, Dong-Ho and Shin, Hakdong and Kim, Hae-Yeong and Kim, Wooki and Ryu, Sangryeol and Park, Cheon-Seok}
}
@article {591,
	title = {Bioremediation. Anaerobes to the rescue.},
	journal = {Science},
	volume = {293},
	year = {2001},
	month = {2001 Aug 24},
	pages = {1444-6},
	keywords = {Bacteria, Aerobic, Bacteria, Anaerobic, Biodegradation, Environmental, Euryarchaeota, Ferric Compounds, Genome, Archaeal, Genome, Bacterial, Hydrocarbons, Aromatic, Hydrocarbons, Chlorinated, Metals, Methane, Nitrates, Oxidation-Reduction, Oxygen, Soil Microbiology, Sulfates, Sulfur-Reducing Bacteria, Water Pollutants, Chemical},
	issn = {0036-8075},
	doi = {10.1126/science.1063294},
	author = {Lovley, D R}
}
