@article {3046,
	title = {Engineering Geobacter pili to produce metal:organic filaments.},
	journal = {Biosens Bioelectron},
	volume = {222},
	year = {2023},
	month = {2023 Feb 15},
	pages = {114993},
	abstract = {<p>The organized self-assembly of conductive biological structures holds promise for creating new bioelectronic devices. In particular, Geobacter sulfurreducens type IVa pili have proven to be a versatile material for fabricating protein nanowire-based devices. To scale the production of conductive pili, we designed a strain of Shewanella oneidensis that heterologously expressed abundant, conductive Geobacter pili when grown aerobically in liquid culture. S. oneidensis expressing a cysteine-modified pilin, designed to enhance the capability to bind to gold, generated conductive pili that self-assembled into biohybrid filaments in the presence of gold nanoparticles. Elemental composition analysis confirmed the filament-metal interactions within the structures, which were several orders of magnitude larger than previously described metal:organic filaments. The results demonstrate that the S. oneidensis chassis significantly advances the possibilities for facile conductive protein nanowire design and fabrication.</p>},
	keywords = {Biosensing Techniques, Electron Transport, Fimbriae, Bacterial, Geobacter, Gold, Metal Nanoparticles},
	issn = {1873-4235},
	doi = {10.1016/j.bios.2022.114993},
	author = {Szmuc, Eric and Walker, David J F and Kireev, Dmitry and Akinwande, Deji and Lovley, Derek R and Keitz, Benjamin and Ellington, Andrew}
}
@article {487,
	title = {Electricity generation by Geobacter sulfurreducens attached to gold electrodes.},
	journal = {Langmuir},
	volume = {24},
	year = {2008},
	month = {2008 Apr 15},
	pages = {4376-9},
	abstract = {The versatility of gold for electrode manufacture suggests that it could be an ideal material for some microbial fuel cell applications. However, previous studies have suggested that microorganisms that readily transfer electrons to graphite do not transfer electrons to gold. Investigations with Geobacter sulfurreducens demonstrated that it could grow on gold anodes producing current nearly as effectively as with graphite anodes. Current production was associated with the development of G. sulfurreducens biofilms up to 40 microm thick. No current was produced if pilA, the gene for the structural protein of the conductive pili of G. sulfurreducens, was deleted. The finding that gold is a suitable anode material for microbial fuel cells offers expanded possibilities for the construction of microbial fuel cells and the electrochemical analysis of microbe-electrode interactions.},
	keywords = {Electrodes, Electrons, Geobacter, Gold, Microscopy, Confocal, Microscopy, Electron, Scanning, Surface Properties},
	issn = {0743-7463},
	doi = {10.1021/la703469y},
	author = {Richter, Hanno and McCarthy, Kevin and Nevin, Kelly P and Johnson, Jessica P and Rotello, Vincent M and Lovley, Derek R}
}
@article {594,
	title = {Reductive precipitation of gold by dissimilatory Fe(III)-reducing bacteria and archaea.},
	journal = {Appl Environ Microbiol},
	volume = {67},
	year = {2001},
	month = {2001 Jul},
	pages = {3275-9},
	abstract = {Studies with a diversity of hyperthermophilic and mesophilic dissimilatory Fe(III)-reducing Bacteria and Archaea demonstrated that some of these organisms are capable of precipitating gold by reducing Au(III) to Au(0) with hydrogen as the electron donor. These studies suggest that models for the formation of gold deposits in both hydrothermal and cooler environments should consider the possibility that dissimilatory metal-reducing microorganisms can reductively precipitate gold from solution.},
	keywords = {Archaea, Bacteria, Chemical Precipitation, Ferric Compounds, Gold, Oxidation-Reduction},
	issn = {0099-2240},
	doi = {10.1128/AEM.67.7.3275-3279.2001},
	author = {Kashefi, K and Tor, J M and Nevin, K P and Lovley, D R}
}
