@article {731,
	title = {Biotechnological potential of aquatic plant-microbe interactions.},
	journal = {Curr Opin Biotechnol},
	volume = {21},
	year = {2010},
	month = {2010 Jun},
	pages = {339-45},
	abstract = {The rhizosphere in terrestrial systems is the region of soil surrounding plant roots where there is increased microbial activity; in aquatic plants, this definition may be less clear because of diffusion of nutrients in water, but there is still a zone of influence by plant roots in this environment [1]. Within that zone chemical conditions differ from those of the surrounding environment as a consequence of a range of processes that were induced either directly by the activity of plant roots or by the activity of rhizosphere microflora. Recently, there are a number of new studies related to rhizospheres of aquatic plants and specifically their increased potential for remediation of contaminants, especially remediation of metals through aquatic plant-microbial interaction.},
	keywords = {Biodegradation, Environmental, Biotechnology, Hydrogen-Ion Concentration, Plant Roots, Plants},
	issn = {1879-0429},
	doi = {10.1016/j.copbio.2010.04.004},
	author = {Stout, L and N{\"u}sslein, K}
}
@article {755,
	title = {Expression and transfer of engineered catabolic pathways harbored by Pseudomonas spp. introduced into activated sludge microcosms.},
	journal = {Appl Environ Microbiol},
	volume = {58},
	year = {1992},
	month = {1992 Oct},
	pages = {3380-6},
	abstract = {Two genetically engineered microorganisms (GEMs), Pseudomonas sp. strain B13 FR1(pFRC20P) (FR120) and Pseudomonas putida KT2440(pWWO-EB62) (EB62), were introduced into activated sludge microcosms that had the level of aeration, nutrient makeup, and microbial community structure of activated sludge reactors. FR120 contains an experimentally assembled ortho cleavage route for simultaneous degradation of 3-chlorobenzoate (3CB) and 4-methyl benzoate (4MB); EB62 contains a derivative TOL plasmid-encoded degradative pathway for toluene experimentally evolved so that it additionally processes 4-ethyl benzoate (4EB). Experiments assessed survival of the GEMs, their ability to degrade target substrates, and lateral transfer of plasmid-encoded recombinant DNA. GEMs added at initial densities of 10(6) to 10(7) bacteria per ml of activated sludge declined to stable population densities of 10(4) to 10(5) bacteria per ml. FR120 degraded combinations of 3CB and 4MB (1 mM each) following 3 days of adaptation in the microcosms. Indigenous microorganisms required an 8-day adaptation period before degradation of 4MB was observed; 3CB was degraded only after the concentration of 4MB was much reduced. The indigenous microbial community was killed when both compounds were present at concentrations of 4.0 mM. However, in parallel microcosms containing FR120, the microbial community maintained a normal density of viable cells. Indigenous microbes readily degraded 4EB (2 mM), and EB62 did not significantly increase the observed rate of degradation. In filter matings, transfer of pFRC20P, which specifies mobilization but not transfer functions, from FR120 to P. putida UWC1 was not detectable (< 10(-7) transconjugants per donor cell).(ABSTRACT TRUNCATED AT 250 WORDS)},
	keywords = {Benzoates, Biodegradation, Environmental, Gene Expression Regulation, Bacterial, Genetic Engineering, Industrial Waste, Pseudomonas, Transfection, Waste Disposal, Fluid},
	issn = {0099-2240},
	author = {N{\"u}sslein, K and Maris, D and Timmis, K and Dwyer, D F}
}
