@article {710,
	title = {Extracellular simian virus 40 transmits a signal that promotes virus enclosure within caveolae.},
	journal = {Exp Cell Res},
	volume = {246},
	year = {1999},
	month = {1999 Jan 10},
	pages = {83-90},
	abstract = {It was reported earlier that entry of simian virus 40 (SV40) into cells is promoted by a signal transmitted by the virus from the cell surface and that SV40 enters cells through caveolae. It is shown here that bound SV40 begins to partition into a caveolae-enriched Triton X-100-insoluble membrane fraction at 30 min postadsorption. Maximal levels of SV40 were seen in that fraction at 1 h. The sterol-binding agent nystatin, which selectively disrupts the cholesterol-enriched caveolae-containing membrane microdomain, selectively blocked the SV40-induced signal. This implies that the SV40 signal is transmitted from that membrane microdomain. The tyrosine kinase inhibitor genistein, which was earlier shown to block the SV40-induced signal and infectious entry, did not block the partitioning of SV40 into the detergent-insoluble membrane fraction. This shows that the signal is not required for the translocation of SV40 to the detergent-insoluble membrane and is consistent with the finding that the signal is likely transmitted from that membrane microdomain. However, electron microscopy of the Triton X-100-insoluble membrane fraction showed that genistein caused SV40 particles to accumulate at the annuli or mouths of the caveolae. In contrast, most SV40 particles were found enclosed within caveolae in parallel samples from untreated control cells. Together, these results imply that SV40 initially binds to flat detergent-soluble membrane. The virus then translocates to a caveolae-containing detergent-insoluble membrane microdomain. From the flat portion of that membrane microdomain the virus induces a signal which promotes its entry into caveolae.},
	keywords = {Animals, Blotting, Northern, Blotting, Western, Caveolin 1, Caveolins, Cell Line, Cell Membrane, Cercopithecus aethiops, Cholesterol, Endocytosis, Genes, myc, Genistein, Kidney, Membrane Proteins, Microscopy, Electron, Nystatin, Octoxynol, Signal Transduction, Simian virus 40, Solubility},
	issn = {0014-4827},
	doi = {10.1006/excr.1998.4301},
	author = {Chen, Y and Norkin, L C}
}
@article {705,
	title = {Relationship between expression of epidermal growth factor and simian virus 40 T antigen in a line of transgenic mice.},
	journal = {Transgenic Res},
	volume = {4},
	year = {1995},
	month = {1995 Sep},
	pages = {306-14},
	abstract = {The pattern of expression of the simian virus 40 (SV40) T antigen gene and resultant dysplasia were re-examined in a line of transgenic mice in which the T antigen gene was under the control of the SV40 early promoter. We found that T antigen expression in the kidney, and resulting dysplastic lesions, occurred exclusively in the distal convoluted tubules and the ascending limbs of Henle. Epidermal growth factor (EGF) expression in the kidney of normal mice was similarly immunolocalized. The correlation between high EGF immunoreactivity in normal mouse tissues and T antigen expression in the transgenic counterpart was also seen in the choroid plexus epithelium and in the submandibular glands of male mice. T antigen was not found in the submandibular gland of transgenic females. Similarly, EGF was only rarely detected in the normal female submandibular gland. In contrast to the correlation between T antigen expression in the transgenic mice and EGF expression in the corresponding tissues of the normal mice, within the dysplastic lesions of the transgenic mice EGF expression was severely diminished. Adenocarcinomas of the male submandibular gland from another line of transgenic mice that expresses the Int-1 transgene, showed similarly reduced levels of immunostaining for EGF. Thus, reduced expression of EGF might be a general feature of dysplasia and tumorigenesis in those tissues that normally express EGF.},
	keywords = {Adenocarcinoma, Animals, Antigens, Viral, Tumor, Cattle, Choroid Plexus, Cricetinae, Epidermal Growth Factor, Female, Gene Expression Regulation, Neoplastic, Kidney, Kidney Tubules, Distal, Male, Mice, Mice, Transgenic, Proto-Oncogene Proteins, Simian virus 40, Submandibular Gland, Submandibular Gland Neoplasms, Wnt Proteins, Wnt1 Protein, Zebrafish Proteins},
	issn = {0962-8819},
	author = {Lafond, R E and Giammalvo, J T and Norkin, L C}
}
