@article {3122,
	title = {Identification of genes specifically required for the anaerobic metabolism of benzene in Geobacter metallireducens.},
	journal = {Front Microbiol},
	volume = {5},
	year = {2014},
	month = {2014},
	pages = {245},
	abstract = {<p>Although the biochemical pathways for the anaerobic degradation of many of the hydrocarbon constituents in petroleum reservoirs have been elucidated, the mechanisms for anaerobic activation of benzene, a very stable molecule, are not known. Previous studies have demonstrated that Geobacter metallireducens can anaerobically oxidize benzene to carbon dioxide with Fe(III) as the sole electron acceptor and that phenol is an intermediate in benzene oxidation. In an attempt to identify enzymes that might be involved in the conversion of benzene to phenol, whole-genome gene transcript abundance was compared in cells metabolizing benzene and cells metabolizing phenol. Eleven genes had significantly higher transcript abundance in benzene-metabolizing cells. Five of these genes had annotations suggesting that they did not encode proteins that could be involved in benzene metabolism and were not further studied. Strains were constructed in which one of the remaining six genes was deleted. The strain in which the monocistronic gene Gmet 0232 was deleted metabolized phenol, but not benzene. Transcript abundance of the adjacent monocistronic gene, Gmet 0231, predicted to encode a zinc-containing oxidoreductase, was elevated in cells metabolizing benzene, although not at a statistically significant level. However, deleting Gmet 0231 also yielded a strain that could metabolize phenol, but not benzene. Although homologs of Gmet 0231 and Gmet 0232 are found in microorganisms not known to anaerobically metabolize benzene, the adjacent localization of these genes is unique to G. metallireducens. The discovery of genes that are specifically required for the metabolism of benzene, but not phenol in G. metallireducens is an important step in potentially identifying the mechanisms for anaerobic benzene activation.</p>},
	issn = {1664-302X},
	doi = {10.3389/fmicb.2014.00245},
	author = {Zhang, Tian and Tremblay, Pier-Luc and Chaurasia, Akhilesh K and Smith, Jessica A and Bain, Timothy S and Lovley, Derek R}
}
@article {3139,
	title = {Improved cathode for high efficient microbial-catalyzed reduction in microbial electrosynthesis cells.},
	journal = {Phys Chem Chem Phys},
	volume = {15},
	year = {2013},
	month = {2013 Sep 14},
	pages = {14290-4},
	abstract = {<p>Microbial electrosynthesis cells (MECs) are devices wherein microorganisms can electrochemically interact with electrodes, directly donating or accepting electrons from electrode surfaces. Here, we developed a novel cathode by using nickel nanowires anchored to graphite for the improvement of microbial-catalyzed reduction in MEC cathode chamber. This porous nickel-nanowire-network-coated graphite electrode increased the interfacial area and interfacial interactions between the cathode surface and the microbial biofilm. A 2.3 fold increase in bio-reduction rate over the untreated graphite was observed. Around 282 mM day(-1) m(-2) of acetate resulting from the bio-reduction of carbon dioxide by Sporomusa was produced with 82 {\textpm} 14\% of the electrons consumed being recovered in acetate.</p>},
	keywords = {Biocatalysis, Bioelectric Energy Sources, Biofilms, Carbon Dioxide, Electrochemical Techniques, Electrodes, Graphite, Nanowires, Nickel, Oxidation-Reduction, Veillonellaceae},
	issn = {1463-9084},
	doi = {10.1039/c3cp52697f},
	author = {Nie, Huarong and Zhang, Tian and Cui, Mengmeng and Lu, Haiyun and Lovley, Derek R and Russell, Thomas P}
}
