@article {701,
	title = {Benefits of in-situ synthesized microarrays for analysis of gene expression in understudied microorganisms.},
	journal = {J Microbiol Methods},
	volume = {74},
	year = {2008},
	month = {2008 Jul},
	pages = {26-32},
	abstract = {Although the genome sequences of many microorganisms are now known, whole-genome DNA microarray platforms consisting of PCR amplicon, or oligonucleotide elements printed onto glass slides have been readily available for only a relatively few, highly studied microorganisms. For those microorganisms more recently cultured or studied by fewer investigators it has been difficult to justify the initial time and expense of developing such array platforms especially if only a limited number of gene expression studies are envisioned. However, in-situ synthesized oligonucleotide (ISO) arrays can be inexpensively fabricated on an {\textquoteright}as needed{\textquoteright} basis with a reduced initial investment in time, personnel, resources, and costs. To evaluate the performance of one ISO array platform, gene expression patterns in Geobacter sulfurreducens under nitrogen-fixing conditions were compared with results from quantitative reverse transcriptase PCR (qRT-PCR) and previously published data from a similar experiment using spotted PCR amplicon arrays. There were strong correlations between the results of the ISO arrays and the results from qRT-PCR (r(2)=0.762) and spotted array (r(2)=0.744) analyses. After initial use the ISO arrays could be successfully stripped and reused. The increased flexibility in array design and reusability coupled with a lower initial investment in terms of fabrication time and cost for the ISO arrays suggest that they may be the preferred approach when investigating gene expression in microorganisms, especially when only a few expression studies are required.},
	keywords = {Bacterial Proteins, Computational Biology, Gene Expression, Geobacter, Nitrogen Fixation, Oligonucleotide Array Sequence Analysis, Oligonucleotides, Polymerase Chain Reaction},
	issn = {0167-7012},
	doi = {10.1016/j.mimet.2007.07.004},
	author = {Postier, Bradley and DiDonato, Raymond and Nevin, Kelly P and Liu, Anna and Frank, Bryan and Lovley, Derek and Methe, Barbara A}
}
@article {514,
	title = {Biofilm and nanowire production leads to increased current in Geobacter sulfurreducens fuel cells.},
	journal = {Appl Environ Microbiol},
	volume = {72},
	year = {2006},
	month = {2006 Nov},
	pages = {7345-8},
	abstract = {Geobacter sulfurreducens developed highly structured, multilayer biofilms on the anode surface of a microbial fuel cell converting acetate to electricity. Cells at a distance from the anode remained viable, and there was no decrease in the efficiency of current production as the thickness of the biofilm increased. Genetic studies demonstrated that efficient electron transfer through the biofilm required the presence of electrically conductive pili. These pili may represent an electronic network permeating the biofilm that can promote long-range electrical transfer in an energy-efficient manner, increasing electricity production more than 10-fold.},
	keywords = {Acetates, Bioelectric Energy Sources, Biofilms, Electricity, Electrodes, Electron Transport, Fimbriae, Bacterial, Geobacter, Microscopy, Confocal, Mutation, Nanowires},
	issn = {0099-2240},
	doi = {10.1128/AEM.01444-06},
	author = {Reguera, Gemma and Nevin, Kelly P and Nicoll, Julie S and Covalla, Sean F and Woodard, Trevor L and Lovley, Derek R}
}
