@article {487,
	title = {Electricity generation by Geobacter sulfurreducens attached to gold electrodes.},
	journal = {Langmuir},
	volume = {24},
	year = {2008},
	month = {2008 Apr 15},
	pages = {4376-9},
	abstract = {The versatility of gold for electrode manufacture suggests that it could be an ideal material for some microbial fuel cell applications. However, previous studies have suggested that microorganisms that readily transfer electrons to graphite do not transfer electrons to gold. Investigations with Geobacter sulfurreducens demonstrated that it could grow on gold anodes producing current nearly as effectively as with graphite anodes. Current production was associated with the development of G. sulfurreducens biofilms up to 40 microm thick. No current was produced if pilA, the gene for the structural protein of the conductive pili of G. sulfurreducens, was deleted. The finding that gold is a suitable anode material for microbial fuel cells offers expanded possibilities for the construction of microbial fuel cells and the electrochemical analysis of microbe-electrode interactions.},
	keywords = {Electrodes, Electrons, Geobacter, Gold, Microscopy, Confocal, Microscopy, Electron, Scanning, Surface Properties},
	issn = {0743-7463},
	doi = {10.1021/la703469y},
	author = {Richter, Hanno and McCarthy, Kevin and Nevin, Kelly P and Johnson, Jessica P and Rotello, Vincent M and Lovley, Derek R}
}
@article {475,
	title = {Graphite electrode as a sole electron donor for reductive dechlorination of tetrachlorethene by Geobacter lovleyi.},
	journal = {Appl Environ Microbiol},
	volume = {74},
	year = {2008},
	month = {2008 Oct},
	pages = {5943-7},
	abstract = {The possibility that graphite electrodes can serve as the direct electron donor for microbially catalyzed reductive dechlorination was investigated with Geobacter lovleyi. In an initial evaluation of whether G. lovleyi could interact electronically with graphite electrodes, cells were provided with acetate as the electron donor and an electrode as the sole electron acceptor. Current was produced at levels that were ca. 10-fold lower than those previously reported for Geobacter sulfurreducens under similar conditions, and G. lovleyi anode biofilms were correspondingly thinner. When an electrode poised at -300 mV (versus a standard hydrogen electrode) was provided as the electron donor, G. lovleyi effectively reduced fumarate to succinate. The stoichiometry of electrons consumed to succinate produced was 2:1, the ratio expected if the electrode served as the sole electron donor for fumarate reduction. G. lovleyi effectively reduced tetrachloroethene (PCE) to cis-dichloroethene with a poised electrode as the sole electron donor at rates comparable to those obtained when acetate serves as the electron donor. Cells were less abundant on the electrodes when the electrodes served as an electron donor than when they served as an electron acceptor. PCE was not reduced in controls without cells or when the current supply to cells was interrupted. These results demonstrate that G. lovleyi can use a poised electrode as a direct electron donor for reductive dechlorination of PCE. The ability to colocalize dechlorinating microorganisms with electrodes has several potential advantages for bioremediation of subsurface chlorinated contaminants, especially in source zones where electron donor delivery is challenging and often limits dechlorination.},
	keywords = {Acetic Acid, Biofilms, Biomass, Electricity, Electrodes, Electrons, Ethylene Dichlorides, Fumarates, Geobacter, Graphite, Microscopy, Electron, Scanning, Succinic Acid, Tetrachloroethylene},
	issn = {1098-5336},
	doi = {10.1128/AEM.00961-08},
	author = {Strycharz, Sarah M and Woodard, Trevor L and Johnson, Jessica P and Nevin, Kelly P and Sanford, Robert A and L{\"o}ffler, Frank E and Lovley, Derek R}
}
@article {540,
	title = {Evidence for involvement of an electron shuttle in electricity generation by Geothrix fermentans.},
	journal = {Appl Environ Microbiol},
	volume = {71},
	year = {2005},
	month = {2005 Apr},
	pages = {2186-9},
	abstract = {In experiments performed using graphite electrodes poised by a potentiostat (+200 mV versus Ag/AgCl) or in a microbial fuel cell (with oxygen as the electron acceptor), the Fe(III)-reducing organism Geothrix fermentans conserved energy to support growth by coupling the complete oxidation of acetate to reduction of a graphite electrode. Other organic compounds, such as lactate, malate, propionate, and succinate as well as components of peptone and yeast extract, were utilized for electricity production. However, electrical characteristics and the results of shuttling assays indicated that unlike previously described electrode-reducing microorganisms, G. fermentans produced a compound that promoted electrode reduction. This is the first report of complete oxidation of organic compounds linked to electrode reduction by an isolate outside of the Proteobacteria.},
	keywords = {Acetates, Bacteria, Bioelectric Energy Sources, Electricity, Electrodes, Electron Transport, Graphite, Microscopy, Electron, Scanning, Oxidation-Reduction},
	issn = {0099-2240},
	doi = {10.1128/AEM.71.4.2186-2189.2005},
	author = {Bond, Daniel R and Lovley, Derek R}
}
@article {552,
	title = {Graphite electrodes as electron donors for anaerobic respiration.},
	journal = {Environ Microbiol},
	volume = {6},
	year = {2004},
	month = {2004 Jun},
	pages = {596-604},
	abstract = {It has been demonstrated previously that Geobacter species can transfer electrons directly to electrodes. In order to determine whether electrodes could serve as electron donors for microbial respiration, enrichment cultures were established from a sediment inoculum with a potentiostat-poised graphite electrode as the sole electron donor and nitrate as the electron acceptor. Nitrate was reduced to nitrite with the consumption of electrical current. The stoichiometry of electron and nitrate consumption and nitrite accumulation were consistent with the electrode serving as the sole electron donor for nitrate reduction. Analysis of 16 rRNA gene sequences demonstrated that the electrodes supplied with current were specifically enriched in microorganisms with sequences most closely related to the sequences of known Geobacter species. A pure culture of Geobacter metallireducens was shown to reduce nitrate to nitrite with the electrode as the sole electron donor with the expected stoichiometry of electron consumption. Cells attached to the electrode appeared to be responsible for the nitrate reduction. Attached cells of Geobacter sulfurreducens reduced fumarate to succinate with the electrode as an electron donor. These results demonstrate for the first time that electrodes may serve as a direct electron donor for anaerobic respiration. This finding has implications for the harvesting of electricity from anaerobic sediments and the bioremediation of oxidized contaminants.},
	keywords = {Anaerobiosis, Electrodes, Electrons, Fumarates, Geobacter, Geologic Sediments, Graphite, Kinetics, Microscopy, Electron, Scanning, Nitrates, Phylogeny, RNA, Ribosomal, 16S, Sequence Analysis, DNA},
	issn = {1462-2912},
	doi = {10.1111/j.1462-2920.2004.00593.x},
	author = {Gregory, Kelvin B and Bond, Daniel R and Lovley, Derek R}
}
@article {573,
	title = {Electricity production by Geobacter sulfurreducens attached to electrodes.},
	journal = {Appl Environ Microbiol},
	volume = {69},
	year = {2003},
	month = {2003 Mar},
	pages = {1548-55},
	abstract = {Previous studies have suggested that members of the Geobacteraceae can use electrodes as electron acceptors for anaerobic respiration. In order to better understand this electron transfer process for energy production, Geobacter sulfurreducens was inoculated into chambers in which a graphite electrode served as the sole electron acceptor and acetate or hydrogen was the electron donor. The electron-accepting electrodes were maintained at oxidizing potentials by connecting them to similar electrodes in oxygenated medium (fuel cells) or to potentiostats that poised electrodes at +0.2 V versus an Ag/AgCl reference electrode (poised potential). When a small inoculum of G. sulfurreducens was introduced into electrode-containing chambers, electrical current production was dependent upon oxidation of acetate to carbon dioxide and increased exponentially, indicating for the first time that electrode reduction supported the growth of this organism. When the medium was replaced with an anaerobic buffer lacking nutrients required for growth, acetate-dependent electrical current production was unaffected and cells attached to these electrodes continued to generate electrical current for weeks. This represents the first report of microbial electricity production solely by cells attached to an electrode. Electrode-attached cells completely oxidized acetate to levels below detection (<10 micro M), and hydrogen was metabolized to a threshold of 3 Pa. The rates of electron transfer to electrodes (0.21 to 1.2 micro mol of electrons/mg of protein/min) were similar to those observed for respiration with Fe(III) citrate as the electron acceptor (E(o){\textquoteright} =+0.37 V). The production of current in microbial fuel cell (65 mA/m(2) of electrode surface) or poised-potential (163 to 1,143 mA/m(2)) mode was greater than what has been reported for other microbial systems, even those that employed higher cell densities and electron-shuttling compounds. Since acetate was completely oxidized, the efficiency of conversion of organic electron donor to electricity was significantly higher than in previously described microbial fuel cells. These results suggest that the effectiveness of microbial fuel cells can be increased with organisms such as G. sulfurreducens that can attach to electrodes and remain viable for long periods of time while completely oxidizing organic substrates with quantitative transfer of electrons to an electrode.},
	keywords = {Culture Media, Deltaproteobacteria, Electricity, Electrodes, Electron Transport, Microscopy, Electron, Scanning, Oxidation-Reduction},
	issn = {0099-2240},
	author = {Bond, Daniel R and Lovley, Derek R}
}
