@article {1567,
	title = {Characterization of a novel facultative Methylocystis species capable of growth on methane, acetate and ethanol.},
	journal = {Environ Microbiol Rep},
	volume = {3},
	year = {2011},
	month = {2011 Apr},
	pages = {174-81},
	abstract = {<p>A non-motile strain of Methylocystis, strain SB2, isolated from a spring bog in southeast Michigan, had a curved rod morphology with a typical type II intracytoplasmic membrane system. This organism expressed the membrane-bound or particulate methane monooxygenase (pMMO) as well as a chalkophore with high affinity for copper and did not express the cytoplasmic or soluble methane monooxygenase (sMMO). Strain SB2 was found to grow within the pH range of 6-9, with optimal growth at 6.8. Growth was observed at temperatures ranging between 10{\textdegree}C and 30{\textdegree}C, with no growth at 37{\textdegree}C. The DNA G+C content was 62.9  mol\%. Predominant fatty acids were 18:1ω7c (72.7\%) and 18:1ω9c (24\%) when grown on methane. Phylogenetic comparisons based on both pmoA and 16S rRNA sequences indicated that this organism belonged to the Methylocystis genus, and was closely related to Methylocystis rosea SV97(T) and Methylocystis echinoides IMET10491(T) (98\% 16S rRNA gene sequence similarity to both strains). DNA  :  DNA hybridizations indicated that strain SB2 had 70\% similarity with M. rosea SV97(T) . Unlike M. rosea SV97(T) , strain SB2 was able to utilize not only methane for growth, but also ethanol and acetate. Furthermore, the predominant fatty acids in strain SB2 were different from those found in M. rosea SV97(T) , i.e. 54.2\% and 39.7\% of fatty acids are 18:1ω8 and 18:1ω7 in M. rosea SV97(T) , while 18:1ω8 is completely absent in strain SB2.</p>},
	issn = {1758-2229},
	doi = {10.1111/j.1758-2229.2010.00204.x},
	author = {Im, Jeongdae and Lee, Sung-Woo and Yoon, Sukhwan and Dispirito, Alan A and Semrau, Jeremy D}
}
@article {1566,
	title = {Constitutive expression of pMMO by Methylocystis strain SB2 when grown on multi-carbon substrates: implications for biodegradation of chlorinated ethenes.},
	journal = {Environ Microbiol Rep},
	volume = {3},
	year = {2011},
	month = {2011 Apr},
	pages = {182-8},
	abstract = {<p>The particulate methane monooxygenase (pMMO) in Methylocystis strain SB2 was found to be constitutively expressed in the absence of methane when the strain was grown on either acetate or ethanol. Real-time quantitative polymerase chain reaction (PCR) and reverse transcription-PCR showed that the expression of pmoA decreased by one to two orders of magnitude when grown on acetate as compared with growth of strain SB2 on methane. The capability of strain SB2 to degrade a mixture of chlorinated ethenes in the absence of methane was examined to verify the presence and activity of pMMO under acetate-growth conditions as well determine the effectiveness of such conditions for bioremediation. It was found that when strain SB2 was grown on acetate and exposed to 40  {\textmu}M each of trichloroethylene (TCE), trans-dichloroethylene (t-DCE) and vinyl chloride (VC), approximately 30\% of VC and t-DCE was degraded but no appreciable TCE removal was measured after 216  h of incubation. The ability to degrade VC and t-DCE was lost when acetylene was added, confirming that pMMO was responsible for the degradation of these chlorinated ethenes by Methylocystis strain SB2 when the strain was grown on acetate.</p>},
	issn = {1758-2229},
	doi = {10.1111/j.1758-2229.2010.00205.x},
	author = {Yoon, Sukhwan and Im, Jeongdae and Bandow, Nathan and Dispirito, Alan A and Semrau, Jeremy D}
}
