@article {1447,
	title = {Mycobacterial Esx-3 is required for mycobactin-mediated iron acquisition.},
	journal = {Proc Natl Acad Sci U S A},
	volume = {106},
	year = {2009},
	month = {2009 Nov 3},
	pages = {18792-7},
	abstract = {<p>The Esx secretion pathway is conserved across Gram-positive bacteria. Esx-1, the best-characterized system, is required for virulence of Mycobacterium tuberculosis, although its precise function during infection remains unclear. Esx-3, a paralogous system present in all mycobacterial species, is required for growth in vitro. Here, we demonstrate that mycobacteria lacking Esx-3 are defective in acquiring iron. To compete for the limited iron available in the host and the environment, these organisms use mycobactin, high-affinity iron-binding molecules. In the absence of Esx-3, mycobacteria synthesize mycobactin but are unable to use the bound iron and are impaired severely for growth during macrophage infection. Mycobacteria thus require a specialized secretion system for acquiring iron from siderophores.</p>},
	keywords = {Animals, Bacterial Proteins, Genome, Bacterial, Iron, Macrophages, Mice, Mutation, Mycobacterium, Mycobacterium Infections, Oxazoles, Protein Binding, Secretory Pathway, Siderophores, Transcription, Genetic, Up-Regulation},
	issn = {1091-6490},
	doi = {10.1073/pnas.0900589106},
	author = {Siegrist, M Sloan and Unnikrishnan, Meera and McConnell, Matthew J and Borowsky, Mark and Cheng, Tan-Yun and Siddiqi, Noman and Fortune, Sarah M and Moody, D Branch and Rubin, Eric J}
}
@article {1448,
	title = {Phage transposon mutagenesis.},
	journal = {Methods Mol Biol},
	volume = {465},
	year = {2009},
	month = {2009},
	pages = {311-23},
	abstract = {<p>Phage transduction is an attractive method of genetic manipulation in mycobacteria. PhiMycoMarT7 is well suited for transposon mutagenesis as it is temperature sensitive for replication and contains T7 promoters that promote transcription, a highly active transposase gene, and an Escherichia coli oriR6 K origin of replication. Mycobacterial transposon mutant libraries produced by PhiMycoMarT7 transduction are amenable to both forward and reverse genetic studies. In this protocol, we detail the preparation of PhiMycoMarT7, including a description of the phage, reconstitution of the phage, purification of plaques, preparation of phage stock, and titering of phage stock. We then describe the transduction procedure and finally outline the isolation of individual transposon mutants.</p>},
	keywords = {Bacteriophages, DNA Transposable Elements, Mutagenesis, Mutation, Mycobacterium, Transduction, Genetic},
	issn = {1940-6029},
	doi = {10.1007/978-1-59745-207-6_21},
	author = {Siegrist, M Sloan and Rubin, Eric J}
}
