@article {1437,
	title = {Illumination of growth, division and secretion by metabolic labeling of the bacterial cell surface.},
	journal = {FEMS Microbiol Rev},
	volume = {39},
	year = {2015},
	month = {2015 Mar},
	pages = {184-202},
	abstract = {<p>The cell surface is the essential interface between a bacterium and its surroundings. Composed primarily of molecules that are not directly genetically encoded, this highly dynamic structure accommodates the basic cellular processes of growth and division as well as the transport of molecules between the cytoplasm and the extracellular milieu. In this review, we describe aspects of bacterial growth, division and secretion that have recently been uncovered by metabolic labeling of the cell envelope. Metabolite derivatives can be used to label a variety of macromolecules, from proteins to non-genetically-encoded glycans and lipids. The embedded metabolite enables precise tracking in time and space, and the versatility of newer chemoselective detection methods offers the ability to execute multiple experiments concurrently. In addition to reviewing the discoveries enabled by metabolic labeling of the bacterial cell envelope, we also discuss the potential of these techniques for translational applications. Finally, we offer some guidelines for implementing this emerging technology.</p>
},
	issn = {1574-6976},
	doi = {10.1093/femsre/fuu012},
	author = {Siegrist, M Sloan and Swarts, Benjamin M and Fox, Douglas M and Lim, Shion An and Bertozzi, Carolyn R}
}
@article {1442,
	title = {Imaging bacterial peptidoglycan with near-infrared fluorogenic azide probes.},
	journal = {Proc Natl Acad Sci U S A},
	volume = {111},
	year = {2014},
	month = {2014 Apr 15},
	pages = {5456-61},
	abstract = {<p>Fluorescent probes designed for activation by bioorthogonal chemistry have enabled the visualization of biomolecules in living systems. Such activatable probes with near-infrared (NIR) emission would be ideal for in vivo imaging but have proven difficult to engineer. We present the development of NIR fluorogenic azide probes based on the Si-rhodamine scaffold that undergo a fluorescence enhancement of up to 48-fold upon reaction with terminal or strained alkynes. We used the probes for mammalian cell surface imaging and, in conjunction with a new class of cyclooctyne D-amino acids, for visualization of bacterial peptidoglycan without the need to wash away unreacted probe.</p>},
	keywords = {Azides, Fluorescent Dyes, Molecular Imaging, Molecular Probe Techniques, Molecular Structure, Peptidoglycan},
	issn = {1091-6490},
	doi = {10.1073/pnas.1322727111},
	author = {Shieh, Peyton and Siegrist, M Sloan and Cullen, Andrew J and Bertozzi, Carolyn R}
}
