@article {1437,
	title = {Illumination of growth, division and secretion by metabolic labeling of the bacterial cell surface.},
	journal = {FEMS Microbiol Rev},
	volume = {39},
	year = {2015},
	month = {2015 Mar},
	pages = {184-202},
	abstract = {<p>The cell surface is the essential interface between a bacterium and its surroundings. Composed primarily of molecules that are not directly genetically encoded, this highly dynamic structure accommodates the basic cellular processes of growth and division as well as the transport of molecules between the cytoplasm and the extracellular milieu. In this review, we describe aspects of bacterial growth, division and secretion that have recently been uncovered by metabolic labeling of the cell envelope. Metabolite derivatives can be used to label a variety of macromolecules, from proteins to non-genetically-encoded glycans and lipids. The embedded metabolite enables precise tracking in time and space, and the versatility of newer chemoselective detection methods offers the ability to execute multiple experiments concurrently. In addition to reviewing the discoveries enabled by metabolic labeling of the bacterial cell envelope, we also discuss the potential of these techniques for translational applications. Finally, we offer some guidelines for implementing this emerging technology.</p>
},
	issn = {1574-6976},
	doi = {10.1093/femsre/fuu012},
	author = {Siegrist, M Sloan and Swarts, Benjamin M and Fox, Douglas M and Lim, Shion An and Bertozzi, Carolyn R}
}
@article {1446,
	title = {Probing the mycobacterial trehalome with bioorthogonal chemistry.},
	journal = {J Am Chem Soc},
	volume = {134},
	year = {2012},
	month = {2012 Oct 3},
	pages = {16123-6},
	abstract = {<p>Mycobacteria, including the pathogen Mycobacterium tuberculosis, use the non-mammalian disaccharide trehalose as a precursor for essential cell-wall glycolipids and other metabolites. Here we describe a strategy for exploiting trehalose metabolic pathways to label glycolipids in mycobacteria with azide-modified trehalose (TreAz) analogues. Subsequent bioorthogonal ligation with alkyne-functionalized probes enabled detection and visualization of cell-surface glycolipids. Characterization of the metabolic fates of four TreAz analogues revealed unique labeling routes that can be harnessed for pathway-targeted investigation of the mycobacterial trehalome.</p>},
	keywords = {Alkynes, Azides, Fluorescent Dyes, Glycolipids, Mycobacterium, Trehalose},
	issn = {1520-5126},
	doi = {10.1021/ja3062419},
	author = {Swarts, Benjamin M and Holsclaw, Cynthia M and Jewett, John C and Alber, Marina and Fox, Douglas M and Siegrist, M Sloan and Leary, Julie A and Kalscheuer, Rainer and Bertozzi, Carolyn R}
}
